HIGHEST-RANKED RESEARCH AND SCHOLARLY ACHIEVEMENTS IN THE LAST FIVE YEARS

Seroprevalence and Genomic Epidemiological Microbial Contributions for Tailored Vaccines Development in Infectious Diseases Global Pandemic Planning

Importance

Seroprevalence and genomic epidemiological studies on Respiratory Tract viruses (RTIs), Human Papillomavirus (HPV), Zika virus, and Rubella virus play a pivotal role in addressing critical public health concerns. These investigations are essential for understanding the seroprevalence and genotypes of these viruses, enabling targeted interventions, and vaccine design, and informing real-time preventive strategies and control.

Impact

We documented 37 patents and novel contributions as well as 82 genomic microbial contributions to the NCBI, USA. Prevalence and dominance of respiratory viruses were reported among over 80% of children below the age of 5 years in Nigeria. Amongst the identified viruses included Respiratory Syncytial virus, Adenovirus, Para-Influenza virus, and Coronavirus OC 229 E/NL63 for the first time in Nigeria which contributed to the understanding of the prevalence and impact of the viruses on treatment approach. Unveiling the genomic epidemiology of HPV contributes to understanding prevalence rates and identifying specific genotypes which is crucial for developing tailored vaccines and screening programs. The identification of the high-risk genotypes of HPV 31 and 35 not covered by the existing vaccines was a significant advancement in the understanding and treatment of cervical cancer in Nigerian women. We offer valuable insights for developing targeted prevention strategies, addressing Female Genital Mutilation, and exploring novel therapeutic approaches. We opined the efficacy of Bridelia ferruginea nanoparticles in inducing apoptosis and beneficial gene modulation in cervical cancer cells as well as Schiff base polyphenols exhibition of anticancer activities. We optimized the molecular detection protocols for Zika virus among serologically positive respondents with contributions of new strains in circulation from Nigeria thereby addressing the challenges of viral confirmation as well as identifying the associated significant risk factors for public health interventions. These contributions would further guide targeted healthcare interventions, vaccine design, and vaccination strategies.

Relevant Output

Published articles on Coronavirus provided good insights into curtailing the transmission in Nigeria and Africa region. A diverse array of 19 HPV genotypes (12 high-risk and 7 low-risk) among Nigerian women was reported which is crucial for developing region-specific vaccines and screening programs. Anticancer capabilities of iron nanoparticles produced from the Bridelia ferruginea plant and synthetic Schiff base polyphenols are a major breakthrough paving the way for the development of more novel therapeutic agents to effectively combat cervical cancer. Confirmation of Zika virus using optimized primers from multiple lineages is therefore advocated to overcome the challenges of false negativity from serological analysis. Also, the primer design for diagnosis of rubella virus using the NSP200 was confirmed revealing the replicative state of rubella infection in pregnant women for appropriate intervention. Genetic sequences of viral strains in Nigeria were deposited in the NCBI genomic bank to support the global surveillance of viruses of public health interest influencing policies, vaccination programs, and healthcare practices (several media interviews, UNILORIN Bulletin, Journals, Channels TV, Press releases and Newspaper blog).

Wastewater Epidemiology and Low Dosage Novel Bioflocculants [CAMATINFECTANT 1, 2 & 3] treatment.

Importance

We documented in Nigeria the epidemiological data of wastewater microflora to efficiently map the pathogenic (particularly polioviruses) bio-community of the immediate environment to curb community transmission and address population immunity gaps from vaccination. Also, the undesirable human and environmental implications of chemical and synthetic flocculants in the process of wastewater treatment has warranted the need for safer and sustainable options that are potent, and non-toxic to both humans and the environment. CAMATINFECTANT 1, 2 & and 3 are biologically produced macromolecules that perform similar function using the principle of flocculation. It has a high Biological Oxygen Demand (BOD5) removal efficiency of 97% and minimum flocculating activity of 62%. Unlike the currently used chemical flocculants (Aluminium sulfate and Ferric chloride) that require a minimum of 5 g/ml effective dose, these products have a low-concentration effective-dose application of 0.2 mg/ml and a turn-around dissolution time of 30-50 minutes. It is efficient in flocs formation and sedimentation and dissolves readily upon application with no trace of smell or taste, which is uncommon with chemical flocculants. These novel products extracted from novel bacteria (Kolawole et al., 2019, Kolawole et al., 2020) have no health-related impact on humans and are biodegradable, eco-friendly, and easily accessible with low cost of production.

Impact

A review of country water and wastewater indicators as well as treatment protocol is hereby advocated to provide real-time data to critical stakeholders for policy formulation and public health intervention via Water Sanitation and Hygiene (WASH) programs. Today, Nigeria uses wastewater sources in communities to track and detect non-polio enteroviruses (NPEV) transmission which often elude clinical diagnosis (AFP) in asymptomatic individuals. Also, CAMATINFECTANT 1, 2 & and 3 will have a significant impact on water resources management and environmental sustainability. Specifically, it will be a valuable substitute for use in wastewater treatment plants which will in turn reduce the concerns for trace chemicals in the treated wastewater before discharge into surface rivers as well as eliminate the presence of chemical-related emerging contaminants in the environment. This is an alternative product that is easy to use with low cost and could be applied in areas with no advanced technology, particularly in low- and medium-income countries where wastewater treatment facilities are costly to establish and expensive to use. Hence, due to its low cost, easy application, and safe usage (to both consumers and the environment), these products will be a suitable alternative to chemical and synthetic flocculants in both developing and developed countries.

Relevant Output

Our research has improved community and national surveillance, awareness, and preparedness for potential outbreaks of bacteria and viruses that have been classified as moderate to high water transmissible pathogens of public health significance by the World Health Organization. The pilot stage of the CAMATINFECTANT 1, 2 & 3 has successfully reached the Technology Readiness Level Four (TRL 4), an international collaboration for product characterization at the University of Warwick, United Kingdom, products exhibited at Federal Ministry of Science and Technology-International Expo 2023, Abuja, Nigeria, several media interviews (UNILORIN Bulletin, Africa Hub Warwick, Press releases and Newspaper blog). Standardization and Optimisation process ongoing, nano-modification products development, scale-up and industrial production with commercialization drives is being planned. Plans are ongoing for submission for patent and journal publication.

FloccuCalc for Computation of Flocculating Activities for Industrial Processes

Importance

Introducing a groundbreaking application designed to revolutionize the determination of flocculating activity in water and wastewater analyses. This cutting-edge tool seamlessly integrates an embedded formula for precise calculations. The user-friendly interface allows researchers to effortlessly control inputs and add samples with a simple click of a button. The application's key feature lies in its dynamic functionality. As users insert control parameters, they can conveniently add multiple samples, streamlining the data input process. Once all relevant samples are incorporated, a single click on the 'Calculate' button triggers the application's artificial intelligence algorithms to swiftly process the data.

Impact

The application presents comprehensive results, enhancing the efficiency of researchers working on flocculants for water and wastewater treatment. The output reveals the percentage of flocculating activity, providing a clear quantitative measure. Additionally, the tool automatically computes the mean, standard deviation, variance, and ANOVA, offering researchers valuable statistical insights into the performance of flocculant samples.

Relevant Output

This innovative calculator serves as an indispensable asset for researchers seeking to streamline and enhance the accuracy of flocculating activity assessments. By amalgamating advanced computational capabilities with user-friendly design, the application empowers researchers in the field of water and wastewater analyses, contributing to advancements in environmental science and wastewater reuse. Plans are ongoing for submission for patent and journal publication.